Middle Littleton Tythe Barn, also known as Middle Littleton Tithe Barn, is a grade I listed 12th or 13th-century tithe barn in the village of Middle Littleton, near Evesham in Worcestershire. It is one of the largest and most notable tithe barns in England. The barn is constructed of a mixture of Blue Lias and Cotswold stones, with a stone tile roof. It was originally built for Evesham Abbey, which was the third largest abbey in England. It is now owned and operated by the National Trust.